mouse anti calnexin99a Search Results


mouse anti cal nexin99a  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank mouse anti cal nexin99a
Mouse Anti Cal Nexin99a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti cal nexin99a/product/Developmental Studies Hybridoma Bank
Average 95 stars, based on 1 article reviews
mouse anti cal nexin99a - by Bioz Stars, 2026-02
95/100 stars
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cnx99a 6 2 1 rrid ab 2722011  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank cnx99a 6 2 1 rrid ab 2722011
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Cnx99a 6 2 1 Rrid Ab 2722011, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cnx99a 6 2 1 rrid ab 2722011/product/Developmental Studies Hybridoma Bank
Average 95 stars, based on 1 article reviews
cnx99a 6 2 1 rrid ab 2722011 - by Bioz Stars, 2026-02
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mouse anti calnexin99a  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc mouse anti calnexin99a
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Mouse Anti Calnexin99a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti calnexin99a/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
mouse anti calnexin99a - by Bioz Stars, 2026-02
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immuno chemistry anti cnx99a  (Developmental Studies Hybridoma Bank)
93
Developmental Studies Hybridoma Bank immuno chemistry anti cnx99a
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Immuno Chemistry Anti Cnx99a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immuno chemistry anti cnx99a/product/Developmental Studies Hybridoma Bank
Average 93 stars, based on 1 article reviews
immuno chemistry anti cnx99a - by Bioz Stars, 2026-02
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mouse anti lamin  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mouse anti lamin
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Mouse Anti Lamin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti lamin/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
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rabbit anti gfp  (Thermo Fisher)
86
Thermo Fisher rabbit anti gfp
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Rabbit Anti Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti gfp/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
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mouse anti golgin 84  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mouse anti golgin 84
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Mouse Anti Golgin 84, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti golgin 84/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
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5a1e  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc 5a1e
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
5a1e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5a1e/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
5a1e - by Bioz Stars, 2026-02
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rabbit anti ha  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti ha
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Rabbit Anti Ha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ha/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
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fluotag-q anti-rfp (n0401)  (NanoTag Biotechnologies GmbH)
90
NanoTag Biotechnologies GmbH fluotag-q anti-rfp (n0401)
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Fluotag Q Anti Rfp (N0401), supplied by NanoTag Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluotag-q anti-rfp (n0401)/product/NanoTag Biotechnologies GmbH
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alexa-405-conjugated goat anti-hrp  (Jackson Immuno)
90
Jackson Immuno alexa-405-conjugated goat anti-hrp
(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), <t>anti-Cnx99A</t> (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .
Alexa 405 Conjugated Goat Anti Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), anti-Cnx99A (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .

Journal: Cell reports

Article Title: Reduced SERCA Function Preferentially Affects Wnt Signaling by Retaining E-Cadherin in the Endoplasmic Reticulum

doi: 10.1016/j.celrep.2018.12.049

Figure Lengend Snippet: (Aand B) Wing disc containing SERCA S5 clones marked bythe absence of RFP (green) stained with anti-Arm (A’ and A”’; red in A) and anti-E-Cad (A” and A””; blue in A). Subapical sections are shown in A-A”, and apical sections in A”’ and A””. Scale bar, 50 μm. (B) z projection through the disc at the position shown by the line in (A), with apical up. In mutant cells, E-Cad colocalizes with Arm below the AJs. (C-F) S2 cells co-transfected with Act-GAL4 and UAS-E-Cad and treated with DMSO (C and D)or10 μM thapsigargin (Tg) (E and F) were stained with anti-E-Cad (C’-F’; green in C-F), phalloidin (C” and E”; red in C-F), anti-Cnx99A (C”’ and E”’; blue in C and E), or anti-Arm (D” and F”; blue in D and F). Scale bar, 5 μM. (G) Quantification of the percentage of cells (means ± SEMs) in which E-Cad is localized at the plasma membrane in Tg- or DMSO-treated cells. ****p < 0.0001 by unpaired t test on three independent experiments. InTg-treated cells, E-Cad does not reach the plasma membrane and colocalizes with Cnx99A. Arm colocalizes with E-Cad in control and Tg-treated cells. (H-K) Wing discs with wild-type (H) or SERCA S5 clones (I) expressing E-Cad RNAi , E-Gad ΔS-αCat clones (J) or SERCA S5 , E-Gad ΔS-αCat clones (K). Clones are positively marked with GFP (green) and stained with anti-Al (H’-K’; red in H-K) and anti-Arm (H”-K”; blue in H-K). E-Cad ΔS-αCat rescuesArm accumulation and al expression in SERCA S5 clones. See also .

Article Snippet: Mouse monoclonal anti-Cnx99A , Developmental Studies Hybridoma Bank , Cat # Cnx99A 6-2-1; RRID AB_2722011.

Techniques: Clone Assay, Staining, Mutagenesis, Transfection, Expressing

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Reduced SERCA Function Preferentially Affects Wnt Signaling by Retaining E-Cadherin in the Endoplasmic Reticulum

doi: 10.1016/j.celrep.2018.12.049

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-Cnx99A , Developmental Studies Hybridoma Bank , Cat # Cnx99A 6-2-1; RRID AB_2722011.

Techniques: Recombinant, Transfection, Plasmid Preparation, Software